Fundamental Rights |  UNESCO Intengible Cultural Heritage From India |  Corruption |  Insectivorous Plants |  Causes of Revolt of 1857 in Points |  Ordinance Making Power of President and Governors |  Mangroves Cover in India |  Coral Reefs in India |  Seasons and Climate in India |  Constitutional and Statutory Bodies in India |  Rock Edicts of Ashoka |  Types of Motions in Parliamentary Procedure |  Types of Majorities in Parliament |  Pressure Groups in World and India |  Order of Precedence |  Types of Rocks |  Treaties in Indian History |  Types of Rocks |  Features of Indian Economy |  Geographical Indications |  Ancient History Terminology and Meanings |  Schedules of the Constitution |  Nuclear Power Plants in India List  |  Types of Clouds for SSC CGL | Nuclear Power Plants in India ListTypes of Ocean CurrentsStructure: Layers of EarthAPPSC Group 1 Answer KeySSC JE 2017 QUESTION PAPEROne Word Substitution for SSC and Bank ExamsMPSC Answer Key 2017 2017Layers of AtmosphereMPSC Answer Key 2017 2017BPSC Solved Question Paper 2017MPPSC Solved Question Paper 2017GST Bill IndiaDelhi SultanateLaw Commission of IndiaOscar Awards Winner 2017 ListDiseases in CropsCommon Drugs and UsesDifferent Types of Deserts in WorldUPSC Civil Services Exam-2016 Answer KeyUPSC CAPF Exam-2016 Answer Key | UPPSC UPPER Subordinate Exam-2016 Answer Key | Regulatory BodiesNational Parks in India | International Airports in India  | United Nation International Years National Highways | National Birds of Countries | 
Types of Speciation |  Unisexual and Bisexual Flowers |  DISANET |  National Child Labour Project Scheme |  Tropical Deciduous Forest |  Hoolock Gibbon: National Parks |  Sectors of Indian Economy |  How Legislative Council is Formed |  Insectivorous Plants |  Causes of Revolt of 1857 in Points |  Ordinance Making Power of President and Governors |  Mangroves Cover in India |  Coral Reefs in India |  Seasons and Climate in India |  Constitutional and Statutory Bodies in India |  Rock Edicts of Ashoka |  Types of Motions in Parliamentary Procedure |  Types of Majorities in Parliament |  Pressure Groups in World and India |  Order of Precedence |  Types of Rocks |  Treaties in Indian History |  Types of Rocks |  Features of Indian Economy |  Geographical Indications |  Ancient History Terminology and Meanings |  Schedules of the Constitution |  Nuclear Power Plants in India List  |  Types of Clouds for SSC CGL | Nuclear Power Plants in India ListTypes of Ocean CurrentsStructure: Layers of EarthAPPSC Group 1 Answer KeySSC JE 2017 QUESTION PAPEROne Word Substitution for SSC and Bank ExamsMPSC Answer Key 2017 2017Layers of AtmosphereMPSC Answer Key 2017 2017BPSC Solved Question Paper 2017MPPSC Solved Question Paper 2017GST Bill IndiaDelhi SultanateLaw Commission of IndiaOscar Awards Winner 2017 ListDiseases in CropsCommon Drugs and UsesDifferent Types of Deserts in WorldUPSC Civil Services Exam-2016 Answer KeyUPSC CAPF Exam-2016 Answer Key | UPPSC UPPER Subordinate Exam-2016 Answer Key | Regulatory BodiesNational Parks in India |
Animals and their Baby Names |  Veto Power of President & Types of Veto |  Ordinance Making Power of President and Governors |  Mangroves Cover in India |  Coral Reefs in India |  Seasons and Climate in India |  Constitutional and Statutory Bodies in India |  Rock Edicts of Ashoka |  Types of Motions in Parliamentary Procedure |  Types of Majorities in Parliament |  Pressure Groups in World and India |  Order of Precedence |  Types of Rocks |  Treaties in Indian History |  Types of Rocks |  Features of Indian Economy |  Geographical Indications |  Ancient History Terminology and Meanings |  Schedules of the Constitution |  Nuclear Power Plants in India List  |  Types of Clouds for SSC CGL | Nuclear Power Plants in India ListTypes of Ocean CurrentsStructure: Layers of EarthAPPSC Group 1 Answer KeySSC JE 2017 QUESTION PAPEROne Word Substitution for SSC and Bank ExamsMPSC Answer Key 2017 2017Layers of AtmosphereMPSC Answer Key 2017 2017BPSC Solved Question Paper 2017MPPSC Solved Question Paper 2017GST Bill IndiaDelhi SultanateLaw Commission of IndiaOscar Awards Winner 2017 ListDiseases in CropsCommon Drugs and UsesDifferent Types of Deserts in World

Sunday, 2 June 2019

What is Cas9 protein that is often mentioned in news?

  By GK Planet Team       Sunday, 2 June 2019
Q. What is Cas9 protein that is often mentioned in the news?
  1. A molecular scissors used in targeted gene editing
  2. A biosensor used in the accurate detection of pathogens in patients
  3. A gene that makes plants pest-resistant
  4. A herbicidal substance synthesized in genetically modified crops
Answer: A molecular scissors used in targeted gene editing

Cas9 (CRISPR associated protein 9)

Cas9 (CRISPR associated protein 9) is a protein which plays a vital role in the immunological defense of certain bacteria against DNA viruses, and which is heavily utilized in genetic engineering applications. Its main function is to cut DNA and therefore it can alter a cell's genome.
Cas9 is a bacterial RNA-guided endonuclease that uses base pairing to recognize and cleave target DNAs with complementarity to the guide RNA. The programmable sequence specificity of Cas9 has been harnessed for genome editing and gene expression control in many organisms. Here, we describe protocols for the heterologous expression and purification of recombinant Cas9 protein and for in vitro transcription of guide RNAs. We describe in vitro reconstitution of the Cas9–guide RNA ribonucleoprotein complex and its use in endonuclease activity assays. The methods outlined here enable mechanistic characterization of the RNA-guided DNA cleavage activity of Cas9 and may assist in the further development of the enzyme for genetic engineering applications.
logoblog

Thanks for reading What is Cas9 protein that is often mentioned in news?

←Previous
« Prev Post
Next→
Next Post »

No comments:

Post a Comment

What you have to say about this?

Recent Posts ⌚

Video Lectures